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However, since unambiguous lineage assignment was still possible by means of this fragment (Additional file 2: Table S2; Figure 1B) we included these specimens in further statistical analyses.
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Generated data are limited to few specimens and further future in-depth investigation of the fluctuations of protein signaling and post translational modifications is needed.
Consequently, the sensitivity of FT MTB for paucibacillary specimens should be re-assessed in further, preferably multi-centre studies with higher numbers of smear-negative TB samples.
Future analysis of α-Gal A deficiency with respect to age, as well as rigorous biochemical and stereological analysis of brain specimens will all be useful in further delineating the onset and progression of CNS pathophysiology in these mice.
phenotyping: any experiment involving scoring the subject for a trait value; genotyping: any experiment yielding a value of the genetic makeup of the subject; cross or mutation experiment: an assay involving crossing two subjects or mutation to generate new stocks; and field collection: collection of data or specimens in the field for further analysis in the lab.
All specimens were further fixed in formalin and dehydrated in ascending concentrations of ethanol.
To further elucidate the PRKCZ variant(s) expressed in the specimens, a further round of transcriptome-walking was performed, in triplicate, using TSP4 TSP6 designed to exon 81 (Supplementary Table 3).
The expression patterns were consistent with the performance of each biomarker in these specimens, which further validated the RT-PCR procedures established for each biomarker in this study.
In addition, expressions of HSP27, 60, and Prx-2 in GCA specimens were further confirmed by immunohistochemical and western blot analyses.
Therefore, the COI sequences of only 143 Niphargus specimens out of 184 were used in further analyses.
The assay yielded good performance characteristic in terms of overall sensitivity and specificity with a potential weakness regarding sensitivity in smear-negative specimens which needs to be addressed in further investigations.
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