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Animal samples except for Pseudotrapelus sinaitus were obtained from local animal dealers as dead specimens, identified for their species name, and registered to public museums or the Specimen Depository of a university (Table 1).
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The four clades only contain specimens identified as H. rehsteineri except for two GenBank sequences assigned to either "H.
All recognized species formed monophyletic groups with high statistical support, with the exception of the Eurasian Vulture, for which specimens identified as subspecies G. fulvus fulvescens appear closely related to the Himalayan Vulture (G. himalayensis).
For instance, two specimens identified morphologically as P. dulcis were assigned to the P. orientalis genetic pool by the Bayesian clustering.
For Adv, seven specimens identified as positive only by the two-tube assay were shown via sequencing to be Adv C, and 4 were Adv B1.
For example, two specimens identified by their morphology as members of B. macani and B. liebenauae Keffermüller, respectively, were identified as B. vernus using COI-based phylogenetic analysis, suggesting mitochondrial introgression [ 16].
Within each set of antibiotic loads (each group), four specimens were identified for each time period.
If extracts from a specimen can be identified for which deep high-throughput sequencing of mtDNA shows that a single mtDNA genome is present with minimal or absent indication of any additional mtDNA, this shows that the DNA preparation derives from a single individual.
A 5 μm tissue section that was adjacent to the infected specimen identified by histology was processed for MALDI-IMS.
Combining the mechanical properties, experimental phenomena and strain development, beam hinge failure mode can be identified for all specimens.
Construct stiffness and cycles to failure were identified for all specimens.
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