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Investigators in the field need to collect adequate specimens for virus isolation.
In parallel with human epidemiologic studies, visits were made to Durba in May and October 1999 to collect specimens for virus ecostudies.
Specimens for virus isolation were collected from outbreaks in different districts of Uganda, sentinel sites, and Mulago hospital, the national teaching and referral hospital, in Kampala from 2000 to 2002.
As part of routine measles case investigations, urine samples and throat swab specimens for virus isolation were collected along with serum samples (0 12 days after rash onset) from patients across Uganda during 2006 through 2009.
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The etiologic agent was confirmed to be Japanese encephalitis virus by analyzing 326 acute-phase clinical specimens for virus-specific antibodies and viral RNA and by virus isolation.
Wild-type rotavirus strains were detected in 8 (12%) of the 65 specimens with any virus detected, whereas a genotype could not be determined for the remaining 57 (88%) specimens for which virus was detected by qRT-PCR.
Prior to specimen inoculation for virus isolation, 200 μl of the specimen was transferred to a 1.5 ml microtube and stocked at -80°C until application to real-time RT-PCR assay.
Study design: A total of 145 patient specimens for influenza virus detection submitted in either viral transport medium or in sterile containers were evaluated by the three methods.
In 1998, there were in total 19,848 specimens for influenza virus isolations collected from all public hospitals (covering about 95% of all hospital beds) and outpatient clinics in the whole territory of Hong Kong SAR.
Our virology data were obtained from one laboratory which consistently received 100 to 200 specimens for respiratory virus diagnosis each week from the patients admitted with acute respiratory disease and these specimen numbers were driven by clinical need and were not capped in any way.
The virology data were then aggregated into weekly age-specific numbers of positive specimens for each virus.
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