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If our observations are confirmed in a wider variety of biological specimens that are regularly collected in "ethanol jars", this approach can pave the way for different applications, which require scanning the contents of these specimens for target taxa such as pests, pathogens, vectors, and rare or endangered organisms.
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At sites 2 and 3 the sample set was enriched for relevant translocation-positive specimens, including pediatric cases, to challenge the assay sensitivity with multiple independent specimens for every target included in the MMA panel.
Document the number of unsatisfactory specimens for any reason (target is 0%).
In total, 56 Mtb-spiked sputum specimens were made: 6 smear-positive and 1 smear-negative specimens for each laboratory targeted in this evaluation.
Candida species and Morganella morganii were identified in five specimens, but targets for these organisms were not present in the PCR assays.
The presented novel extraction method opens up the possibility to characterise the shorter DNA fragments that previous extraction methods failed to retain, thereby making more ancient specimens possible targets for ancient DNA analysis.
Interestingly, amplification of sequences was detected in a total of 8 specimens, for four of the tested targets: TP63, 5p15.2, EGFR and MYC.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com