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The use of these human tissue specimens for protein expression studies was approved by the local ethics committee of the University Hospital of Cologne, informed consent was obtained from the patients, and the specimens were procured from the centralized Biobank of the Center for Integrated Oncology CIO Cologne-Bonnnn (http://www.cio-koeln-bonn.de/en/medical-professionals/biobank/).
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Given its central role in proapoptotic signaling, we analyzed a series of more than 600 precision-annotated primary RCC specimens for PUMA protein expression.
Specific immunohistochemical staining was performed on HE-negative specimens for the protein S100 and the melanoma-related antigen HMB45.
A < 24 h postmortem time limit allows comparison of necropsy tissue with freshly collected surgical specimens for their protein levels, cell morphology and tissue integrity [ 37, 38].
A <24-h postmortem time limit allows comparison of necropsy tissue with freshly collected surgical specimens for their protein levels, cell morphology, and tissue integrity (Gittins and Harrison, 2004; Stan et al., 2006), as well as mGluR5 immunoreactivity (Notenboom et al., 2006).
Both whole undissected cryostat specimens and LCM-specimens were used for protein identification.
Monitoring of response was performed after each treatment cycle and at study-end by means of quantification of serum immunoglobulins, serum protein electrophoresis, immunofixation and collection of 24-hr urine specimen for total protein, electrophoresis and immunofixation.
For specimens on which protein C, protein S and antithrombin tests were all ordered, 15.7% of these specimens had positive results for two of the three analytes; 1.2% had positive results for all three.
In order to determine the cell types producing galectin-9 in chronic HCV, we analyzed paraffin-embedded liver biopsy and liver resection specimens for galectin-9 protein by immunohistochemistry (IHC).
The number of cells in both specimens (for mRNA and protein analysis) is unknown and cannot be equal.
We therefore tested melanoma primary resection specimens for mRNA and protein expression and PTEN/MMAC1 cDNAs for the presence of mutations or deletions.
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