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PGB conceived the protocol and collected specimens for processing.
TAG conceived the protocol and collected specimens for processing.
To maintain WHO certification as polio-free, Australia is expected to: 1) detect at least one case of non-polio AFP for every 100,000 children less than 15 years of age annually; 2) collect two faecal specimens greater than 24 hours apart and within 14 days of the onset of paralysis, from at least 80% of AFP cases; and 3) submit all specimens for processing to a WHO accredited laboratory [ 6].
Surrounding hospitals in the sub-district also send their sputum specimens for processing at this Hospital.
For pragmatic reasons, duplicate specimens for processing by the PCR assay will only be taken in the RICU in the Belfast HSCT.
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We thank ward staff at the Alfred Hospital for assisting in this survey, microbiology staff including Lisa Liolios for processing specimens and performing vanA and vanB PCR and the staff at the Microbiological Diagnostic Unit (The University of Melbourne) for performing ribotyping.
Finally the total workload was apportioned according to the skills required for processing the specimens at different stages.
We deeply thank Elisabeth Errazuriz and Simone Peyrol (Centre Commun d'Imagerie de Laennec) for processing our specimens for EM analysis, Sylvie Fiorini and Avril Trejdosiewicz for their technical assistance, and Cathy Berthet for her valuable secretarial help.
With regards to laboratory preparedness our survey did not assess the bio containment levels for processing blood specimens for EVD diagnostics.
We also thank the research personnel including Mrs Valerie Logan and Abbey Hamilton for their help with data management and data cleaning, research nurses Clare Wilson, Kobi Schutz, Emma Tilley and Stacey Svenson for their help with data collection and Kim Hare, Vanya Hampton and Estelle Carter for processing the specimens.
The HPTE method is evaluated theoretically by using finite element methods (FEM) and experimentally by using HPTE for processing copper specimen and the results are presented and discussed.
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