We began searching for Chlorobalius leucoviridis katydids during our cicada-collecting trips to obtain specimens for observations, playbacks, and predation trials.
To prepare specimens for SEM observation, the samples were dipped into liquid nitrogen and immediately fractured.
To prepare specimens for TEM observation, SiC nanomaterial was first dispersed in acetone by ultrasonication for 5 min. Then, a drop of the suspension was dipped onto a carbon-coated copper TEM grid.
Aluminum and brass samples were cut from the middle sections of the billets; specimens for metallographic observation were prepared using standard polishing techniques and etched with Keller's reagent (2.5 mL HNO3, 1.5 mL HCl, 1.0 mL HF, and 95 ml water) for aluminum, and ammonium persulfate solution (10% in water) for brass.
Thin specimens for TEM observation were prepared by Ar-ion thinning by the following procedure: the graphene-on-SiC samples were mechanically ground to 70 μm thick, and the center of the sample was thinned by dimple grinding, followed by Ar-ion beam thinning under a low angle of 8° at 5 kV.
Before fabricating specimens for tissue observation from the experimental animals, a mixture of xylazine HCL (2.3 mg/kg, Bayer) and ketamine (5 mg/kg, Yuhan Corporation) was intravenously injected into the minipigs at four, eight, and 12 weeks after graft to put the minipigs under anesthesia.
Specimens for the observation of jaw morphology were also preserved in 10% formaldehyde solution.
Voucher specimens for seed observations are also preserved at TNU. Seeds for scanning electron microscopy (SEM) were collected from mature fruits.
The specimens for TEM observations were prepared by drying droplets of the LPCMO powders from ethanol dispersion onto a holey carbon grid.
Two types of specimens were prepared for observation under electron and light microscopes.
