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As described above, the specimens for light microscopic observations were decalcified with 10% EDTA and embedded in paraffin.
Specimens for light microscopy were stained with haematoxylin and eosin, periodic acid Schiff, Weigert's elastica-van Gieson, Masson trichrome or periodic acid methanamine silver stain according to routine methods.
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The specimen for light microscopy contained eight glomeruli, which was almost unremarkable.
The specimen for light microscopy contained 15 glomeruli; 7 exhibited global sclerosis, 2 severe segmental sclerosis, and 2 exhibited only minor abnormalities.
The biopsy specimen for light microscopy contained 15 glomeruli, of which three were globally sclerosed and one had a segmental sclerotic lesion.
These specimens were processed for light microscopy, following the routine procedures of surgical pathology specimens stained with hematoxylin and eosin, and diagnosed by pathologists from the hospital service.
Specimens were processed for light and electron microscopy.
Thawed specimens were processed for light microscopy and for scanning and transmission electron microscopy, Cell death was determined by the TUNEL method.
The liver specimens were processed for light and electron microscopy.
A renal biopsy was performed and specimens were sent for light, immunofluorescent and electron microscopy.
Morphology is further studied by fixation of selected specimens and preparation for light microscopy and electron microscopy.
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