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Using flow cytometry, we investigated tumour cells from seven dissociated surgical specimens for expression of NG2, nestin, vimentin and CD133.
In our previous study, we had examined the same collection of clinical specimens for expression of CDKN2A and ARF mRNAs with β-actin as a control [ 23] and then we compared the expression levels of these mRNAs.
We scored the gene expression profiles of the normal adjacent breast specimens for expression of a tumorigenic stem-cell signature, and found a strong correlation between high signature manifestation and CD44+CD49f+CD133/2+ stem-cell staining.
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The typical FFPE specimens used for expression analysis in this study are shown in Fig. S1.
Here, we used differential expression of CD90 to isolate viable CD90-expressing stromal cells directly from prostate cancer specimens for gene expression profiling and comparison to normal tissue stromal cells.
To further delineate the correlation between SPHK1 and Bim expression in glioma, we next examined clinical primary glioma specimens for the expression of SPHK1 and Bim.
Next, utilizing a tissue microarray we simultaneously evaluated 107 surgically resected human pancreas cancer specimens for CEA expression with IHC to validate previous reports and compare with our xenograft models.
We have attempted to address this limitation by evaluating patient biopsy specimens for ALDH expression using immunohistochemistry (Figure 4).
Total RNA was extracted from snap frozen tissue specimens for mRNA expression study using real-time quantitative PCR (12, 14).
We also used a quantitative real-time RT-PCR approach, and analyzed all breast tumor specimens for DSC3 expression.
Using a technique that we described recently, 43 HP-positive patients with available tumor specimens for CagA expression were analyzed.
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