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The use of the specimens for construction of the TMA and subsequent biomarker molecular pathway analysis was approved by the Children's National Medical Center IRB and the Armed Forces Institute of Pathology IRB.
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Specimens used for construction of the normalized library (named CCAG) (Table 1) were acclimated or exposed to physiological challenges that included variation in temperature, salinity, hypoxia, starvation, exercise, moult cycle, metals, pesticides and desiccation (Table 1).
Avoid the "for" construction.
The use of clinical specimens for the construction of tissue microarrays (TMAs) was approved by the ethical committee of the University Hospital of Basel, Switzerland.
Representative areas of invasive BCs were selected on hematoxylin and eosin (H&E -stained sections, and two tissue cores (2 mm in diameter) were obtained from eacH&E -stainedor TMA consectionsn andpreviously described [ 16].
We used the GSE2109_breast dataset consisting of 351 breast tumor specimens for co-expression network construction.
Look for untreated specimens at construction sites, community colleges, buildings under renovation, or shipping warehouses and plane them down and/or kiln-dry them in a homemade kiln-dryer to uncover their hidden beauty.
Formalin-fixed, paraffin-embedded tissue blocks of radical prostatectomy specimens were selected according to tissue availability for construction of a TMA.
Harvard miniature compaction test apparatus having 3.1 cm in diameter by 7.1 cm height was used for construction of unconfined compressive strength test specimens.
For construction of the TMA, core needle biopsy specimens were retrieved from the tumour blocks using a manual arrayer (Beecher Instruments, Sun Prairie, WI, USA) and positioned into a recipient paraffin array block.
These approaches, however, relied on organ preparation by dissection of the specimens and the establishing of amino acid sequence information by tandem mass spectrometry for construction of a cladogram.
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