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Across 113 FFPE breast cancer specimens, expression of the PAM50 genes, subtypes and ROR scores were highly concordant between qRT-PCR and nCounter using the PCR-based classifier.
To further identify the basal subtype among these breast cancer specimens, expression of CK5/6 and EGFR was determined in the triple-negative tumors.
In 10 paired specimens, expression of the p27kip1 cyclin-dependent kinase inhibitor was increased from a mean basal level of 3 26.5% and that of CK-1 was increased from 4.7 37.3%.
In 35 cases of normal lung tissues examined by immunohistochemistry, NKD1 was mainly expressed in the cytoplasm of bronchial epithelial cells (≥3 score According to our evaluation criteria, they were categorized as normal expression. In contrast, in the 100 NSCLC specimens, expression of NKD1 was reduced (<3 score) in 78 (78%) samples and was normal (≥3 score) in 22 (22%) samples (Table 2).
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Of the 57 OSCC specimens, the expression of NANOG was strong in 21 specimens, moderate in 18 specimens, and weak or negative in 18 specimens.
BCL6 protein was highly expressed in breast cancer cell lines and tissue specimens and expression of BCL6 protein was associated with disease progression and poor survival of breast cancer patients.
In a different cohort of 122 human breast tumors and 32 normal breast specimens, the expression of CCN3 was found to be lower in tumor tissues when compared to normal specimens [ 79].
In GN specimens, podocyte expression of 72-AA IL-8 varied notably, with the greatest level found in minimal change disease and the lowest level found in acute endocapillary GN.
Significantly, CDKN1C expression was absent or negligible in clinical specimens, enforced expression of CDKN1C in G401 RT cells induced cell cycle arrest and knockdown of endogenous CDKN1C increased proliferation in G401 RT cells as well as attenuating the effects of SMARCB1 re-expression on cell proliferation.
In the same specimens the expression of fibronectin integrin receptors was evaluated.
In CSCs isolated from primary PDAC surgical specimens, the expression of CD95 positively correlates with EMT markers.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com