Following this, the specimens were embedded with Optical Cutting Temperature embedding matrix (Tissue-Tek®, Agar Scientific Ltd ,Essex, UK).
Specimens were embedded with Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA, USA), staining cell nuclei in blue, and photographed using an Olympus AX70 microscope equipped with epifluorescence illumination (Olympus, Tokyo, Japan).
The specimens were either embedded with the luminal side up or in cross section.
The resin is mixed with its catalyst (see Table 1 for ratios) and poured as three separate layers, with the specimen embedded in the middle layer (Fig. 1A F).
The specimen was post-fixed, washed, dehydrated, embedded with SPI812, positioned, sliced, stained, and observed under Hitachi H-7650 transmission electron microscope (Hitachi, Japan).
Liver biopsy specimens fixed in 10% buffered formalin solution were embedded with paraffin.
The specimens were dehydrated through ethanol series (30-50-70-80-90-100%) and EponddEponith Epon.
Experimental investigation was conducted on three 1 3 scaled high strength concrete composite wall (HSCCW) specimens with embedded steel truss with a medium aspect ratio (1.0 3.0) under cyclic load reversals and constant axial load, to examine the influences of steel usages of embedded truss chord and web brace on the overall seismic behavior of the specimens.
Once infiltrated, the specimens were embedded perpendicularly in blocks with the trim edge down, providing selected cross-sectional profiles across the aorta.
