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PTB suspects were included after at least two negative Acid Fast Bacilli (AFB) smears in conventional sputum specimens either at a first line health facility or at the hospital level.
Participants in the surgical control, benign control and case populations donated serum specimens either at a pre-surgical appointment 1 to 39 days prior to surgery or on the day of surgery after administration of anesthesia but before surgical treatment or chemotherapy.
Prior to DNA extractions, we stored all specimens either at -80°C or in 100% ethanol at room temperature.
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For assemblage assessment, bulk samples were taken at each station with the specimens either dried on slides directly or collected as bulk samples in ethanol.
The room now houses specimens either too precious or too damaged to display.
There was no apparent geographical structure among arenosa specimens either.
The proliferative capacity was not decreased relative to controls in specimens obtained either at enrollment or at the end of the study.
Tumour expression of HER2 was determined by immunohistochemical (IHC) analysis using a monoclonal antibody CB11 (Biogenex, San Ramon, CA, dilution 1 : 10, in 19 patients) or a polyclonal antibody DA485 (DAKO Corp., dilution 1 : 100, in six patients) on paraffin-embedded, formalin-fixed tumour specimens collected either at the time of initial biopsy or at recurrence.
tumor specimens were obtained either at laparotomy, laparoscopy or paracentesis of a malignant ascites or effusion.
Voucher specimens are deposited either at the Institute of Botany, University of Innsbruck, Austria (IB), the Faculty of Science, University of Zagreb, Croatia (ZA), the Faculty of Agriculture, University of Zagreb, Croatia (ZAGR), the Faculty of Biology, University of Belgrade, Serbia (BEOU) or the Natural History Museum Belgrade, Serbia (BEO; Table S1).
Specimens were either cured at room temperature or hydro-thermally treated at 75 °C for five hours.
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