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The development of gene array technology has quickly evolved to allow analysis of very small specimens derived by laser capture microdissection or cell fractionation.
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In parallel with these mouse studies, we investigate the roles of mast cells in human health and disease by conducting studies of human mast cells, or by analyzing specimens derived from patients with asthma or other disorders in which mast cell have been implicated.
We therefore performed TUNEL assays on specimens derived from 4 patients affected by NPHP and carrying mutations in the NPHP1 gene.
Subsequently, groups of mice were sacrificed 48 or 120 hours post infection and after semi-daily therapy and viral titers were determined from pooled specimens derived from the nasal cavity and lung by plaque assays.
This study shows for the first time that metastatic CRC specimens derived from patients previously treated with conventional chemotherapy can be lysed by patient T cells.
The expression of CAR was assessed by immunohistochemical staining of tissue microarrays (TMA) containing 3714 specimens derived from 100 malignancies and from 273 normal control tissues.
Kesting et al. have confirmed our observations by reporting overexpression of hBD-3 in oral cancer tissues using paired cancerous and noncancerous specimens derived from 46 patients [20].
The early stage cancer specimens were from radical prostatectomy specimens derived from patients with clinically localized prostate cancer.
Tumor-tissue derived from three resected specimens derived from one conventional and two dedifferentiated chondrosarcomas were used for culture.
Captive wild specimens derive from various German institutions - the majority from 'The Museum of Domesticated Animals', where they were likely grown for 3 to 5 generations, or kept for much longer in captivity before being bought by the institute in 1882.
It should also be noted that while 73PG specimen derived from a small in size tumor, suggestive of an early stage of neoplastic progression, 60CC specimen derived from the largest one, probably associated with a more advance stage of tumor progression characterized by an aberrant and "clear cell" atypical genomic profile [ 2].
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