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Similar data was obtained from frozen unfixed liver specimens, (data not shown).
This semi-nested PCR protocol requires only 10 copies of cDNA template per sample to produce sufficient product for cloning and does not produce nonspecific product from original clinical specimens (data not shown).
Growth of GAS in AF was not restricted to the serotype M28 strain, as serotype M1 strain MGAS5005 grew at a comparable density to the M28 strain in one of the individual growth-positive specimens (data not shown).
Hierarchical clustering using the 39-gene signature and an ovarian cancer training dataset from M.D. Anderson Cancer Center with control/non-malignant and serous EOC revealed a highly similar pattern among patient specimens (data not shown).
Other MAbs did not show any specific immunostaining of tissue specimens (data not shown).
Only nonpathogenic bacteria were cultured from the respiratory specimens (data not shown).
Similar(29)
In only one case (pl.05) the maternal DNA sample did not match with the placental specimen (data not shown), and therefore that maternal DNA was removed from the analysis.
In a pretest, we found that YKL-40 was not ubiquitously expressed by cancers in the same density or intensity; instead, its expression pattern commonly displayed diverse levels in each tissue specimen (data not shown).
Sensitive PCR protocols have been developed and validated to amplify serogroup-specific polysialyltransferase genes, and the variable regions of porA and fetA from culture negative specimen (data not shown).
However, on the other RA synovial specimens examined (data not shown) both CXCR4 and CCR5 expression was abundant, which is consistent with numerous published reports (for review [ 29]).
Neutralizing activity to SARS-CoV was not found in any of the ELISA-positive samples, and RT-PCR did not detect CoV nucleic acid in 262 serum specimens tested (data not shown).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com