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Matched serum and saliva specimens controlled for time of day and collected less than 30 minutes apart were obtained in 28 women with normal singleton pregnancies between 32 and 38 weeks of gestation and in 43 women during the first six months postpartum.
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It was an important feature of our design that specimens were split in the laboratory before comparison, such that comparisons between staining techniques (incremental yield) were made on the same sputum specimen, controlling for between sample variation.
Additionally, we obtained and analyzed dust specimens collected on the site (DS) and examined old specimens (controls for old cases; COC) unrelated to the WTC disaster that were routinely submitted to our laboratory for asbestos burden analysis (n = 40) or obtained for research purposes from autopsy or surgical specimens (n = 20) of patients without history of WTC exposure.
Experimental protocols were used that minimized end-artifact errors and controlled for specimen orientation.
The β2 microglobulin gene amplification was carried out on each vaginal specimen to control for PCR inhibition [16].
For data acquisition, the ultrasound transducer was positioned such that the focal zone was at the same depth in each imaged specimen to control for any potential attenuation.
Collection and testing of this second distinct specimen was requested to control for clerical errors or specimen mix-up.
Left and right femora of one individual were randomized to either Group A (NCB) or Group B (PERILOC), allowing for a pair-wise comparison in an attempt to control for specimen variability.
There were no significant differences between the treated and controlled specimens for teeth treated with 10% CP or 35% HP.
For fixation the following solutions were used: 1. Specimens for control: 5% GA (Serva) buffered with 0.15 M sodium cacodylate, pH 7.4. 2. Specimens for improved contrast with tannic acid: 5% GA buffered with 0.15 M sodium cacodylate, pH 7.4 + 1% tannic acid (Sigma-Aldrich Chemie, München, Germany).
The NIHRD also checks the ribonucleoprotein (RNP) for influenza specimens serving as controls for the QC of specimen collection by the health personnel in the laboratory.
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