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In 17 of 19 specimens cells with upregulated GLUT-1 were also observed in the peripheral (presumably normoxic regions) of some of the intraductal tumours.
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When capturing images, exposure times were held constant between specimens and cells with fluorescent staining selected using the thresholding tool from the NisElements software.
ASC was used for specimens containing cells with cytologic features of squamous intraepithelial lesions.
Total RNA was extracted from tissue specimens and cells with different treatment using TRIzol (Invitrogen) according to the manufacturer's instructions.
The explanation for this discrepancy could be the contamination of analyzed specimens by cells with the wild type P53, combined with mutated P53 mRNA overexpression, and a phenomenon of P53 heterozygous or hemizygous mutations.
The apparatus utilizes the axial Couette flow generated in removable cylinder specimen cell filled with a liquid.
DFA was carried out using specific monoclonal antibodies for the detection and identification of RSV in direct respiratory specimen cell preparation with Light DiagnosticsTM Respiratory Syncytial Virus DFA kit, cat.
The appropriate metric is the proportion of spatial cells in which specimens were found with the parasite or, alternatively, if sufficient statistics may be obtained, ε cells with specimens with parasite | total cells with specimens).
In a separate study, immunohistochemical analysis revealed that 40% of grade III astrocytomas and GBM tumour specimens contained neoplastic cells with macrophage markers (Rossi et al., 1987).
The Giemsa staining of the sorted specimens revealed large cells with expanded cytoplasm containing vacuoles, suggesting that they are of the monocyte-macrophage lineage (Fig. 3b, right photo (iv)).
A specimen cell was held with two glass micropipettes, the tips of which were coated with a urethane resin adhesive.
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