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Current real-time RT-PCR assays allow sensitive detection of SARS-CoV nucleic acid in clinical specimens by targeting N gene sequence, as shown here, or pol gene sequence (11 – 15 ).
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Classification of lung cancer specimens by miRNA target genes was compared to the clustering profiles of mRNA expression in the original article publishing the Database 1 [ 16].
Since the rate of deposition of generated structures is proportional to the number of evaporated particles, denser structures are synthesized when specimens are targeted by higher energy laser pulses.
Second, it serendipitously photo-bleaches the ThS fluorophores present in regions of the specimen targeted by the mask.
SAFV RNA was detected in 12 (3.2%) of 373 fecal specimens by using RT-PCR with primers targeting the 5′ UTR gene.
HRVs were detected in nasopharyngeal swab specimens by seminested reverse transcription PCR (RT-PCR) targeting the 5′-noncoding 5′-noncoding (10 ).
Thirty-two of these undetected targets were found in coinfections, and the remaining 8 targets (2 Adv, 3 RSVB, 2 HRV, and 1 PIV3) were single-target positive specimens by the two-tube assay.
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Specimen blends were prepared targeting approximately 10%, 5%, 2.5%, and 1.25% mutant DNA as quantified by MPP pyrosequencing.
The amount of target DNA was quantified in a sub-sample of specimens by Real Time (RT) PCR.
To determine the clinical significance of miR-183 target genes, we examined PDCD4 mRNA levels in 25 pairs of matched HCC specimens by qRT-PCR.
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