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The Cycadeoidea specimens are viewed as crucial to this project as they may provide new information about the origin of angiosperms.
In year 3 students attend laboratory courses in which microscopic and macroscopic specimens are viewed.
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The specimens were viewed using a Tecnai G2 transmission electron microscopy at 75 keV.
After staining, specimens were viewed under a stereomicroscope (SZX12, Olympus, Japan), and immediately photographed.
Clean sand and silt specimens were viewed under scanning electron microscope (SEM) to see the shape of particles which are shown in Fig. 3.
The specimens were viewed using transmission electron microscopy [TEM] (JEM.1200ex, JEOL Ltd., Herts, England, UK), and images were recorded in digital imaging plates and scanned in a Ditabis Micron scanner (Ditabis AG, Pforzheim, Germany).
The stub containing the sample was placed in the SEM chamber and coated with palladium with an auto-fine coater for 180 seconds; specimens were viewed with a JEOL JSM 6400 SEM Attached to EDX Energy Dispersive X-rayy) at working distance of 22 mm and an accelerating voltage of 15 kV.
The specimens were viewed with an Olympus BX51 microscope at magnification of 40×.
Specimens were viewed under an Olympus IX 50 fluorescence microscope fitted with adequate filter cubes.
The specimens were viewed using a PASCAL 5 confocal laser scanning microscope (Zeiss, Jena, Germany) after a 30 min incubation period at 32°C in the dark.
Specimens were viewed using a Leica microscope equipped with epifluorescence illumination and appropriate fluorescence filters for DAPI, Alexa Fluor 488/GFP and Alexa Fluor 546.
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