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It is important to use both IgM and NS1-antigen tests to confirm acute dengue particularly under the adverse field conditions, where proper storage and transportation of specimens are missing, which substantially reduce the sensitivity of RT-PCR for detecting DENV RNA.
It is important to use both IgM and NS1-antigen tests to confirm acute dengue particularly under the adverse field conditions, where proper storage and transportation of specimens are missing, which substantially reduce the sensitivity of the RT-PCR for detecting DENV RNA.
Similar(58)
This assumes that the proportion of specimens that test positive for recent infection is the same for persons with available specimens and for those whose specimens were missing.
Usually those specimens were missing at least one leg, and often more from routine barcoding analysis.
Approximately 12% of requested specimens were missing or unidentifiable in hospital pathology databases.
A WPV case was defined as an AFP case for whom a stool specimen tested positive for WPV by virology, while a clinical compatible polio case was defined as an AFP case for whom stool specimens were missing or tested negative for WPV, but was determined to be polio-compatible by the provincial Polio Expert Committee of China after the standard 60-day follow-up examination.
The results indicated that two N9-RT-LAMP-positive specimens were missed by RT-PCR.
The Luminex xTAG RVP Fast assay identified 111 specimens to be coinfections, 83 of them (74.77%) were in complete agreement between both assays, and detection of 13 viruses (in 12 specimens) was missed by the two-tube assay.
A brief survey of hospital personnel revealed that specimens were missed mainly because of complications during delivery, premature birth and/or small size of the infant resulting in small cord blood volume, and logistical factors such as understaffing.
After his death in 1991, it was discovered that the specimen was missing and may have been stolen or sold.
The specimen is missing its head and tail, although the rest of the skeleton is mostly intact.
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