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The specimens are fixed in a two axis translation stage for laser irradiation.
Postoperative specimens are fixed with formalin, which induces shrinkage of pathological samples.
The specimens are fixed in buffered formalin and submitted for histological processing and evaluation.
The harvested specimens are fixed in 4% PBS-buffered formaldehyde and decalcified in 10% PBS-buffered EDTA at room temperature for about 2 months.
The methylene blue assisted technique is another approach used for harvesting LNs. 12 In this approach, the specimens are fixed with formalin-containing methylene blue after surgery.
The specimens are fixed with formalin with methylene blue after surgery, by which physicians are easy to pick up LNs stained by blue dye.
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Kidney specimens were fixed in 0.1 mol/L phosphate-buffered Karnovsky's fixative.
Specimens were fixed again overnight at 4 °C.
All specimens were fixed in paraformaldehyde and embedded in paraffin.
In each group, six specimens were fixed with a 3.5-mm locked proximal tibia plate and six specimens were fixed with a 4.5-mm locking plate.
Muscles from 10 fresh cadaver specimens were fixed by high-pressure perfusion using buffered formaldehyde.
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