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Ultra-deep pyrosequencing of KRAS amplicons with GS Junior 454 proved to be a highly sensitive and quantitative technique to analyse somatic mutations in cancer specimens, and which can also be used in a high-throughput assay.
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To ensure that there was a high probability of cancer being present, sections were cut from all four sides of the specimen and which confirmed malignancy.
The class of the omitted specimen was then predicted by computing the value of the compound covariate for the specimen, and which side of the threshold it fell on (that is, which class mean it was closest to) was determined.
To verify the measurement system, an additional trial was carried out in each case (flat versus edge; 6,000 versus 12,000 rpm) using two compact marble blocks (19.7 × 17.3 × 23.1 mm), whose measurements corresponded to those of an average CT specimen and which were equipped with two identical, diagonally drilled holes for the insertion of thermal sensors.
O'Rourke (2007) has provided guidance on the use of human specimens and banking, which could be adapted to other forms of data banking.
In the 12 tumors showing discordance of the IHC score for HER2 expression between the CNB and surgically resected specimens, and for which the FISH assay was successful, judgments for the FISH results were concordant in 11 922%) (Table 5).
Three one-millimeter tissue cores from formalin-fixed paraffin embedded donor blocks were precisely arrayed into a new recipient paraffin block that included tumor specimens and controls, which included multiple cores of normal tissue from 10 different organs.
In my memory, he was always the arbiter of the apple, standing atop a ladder in a pick-your-own orchard, inspecting each specimen and deciding which were good enough for the bushel basket I held below.
He then continued to the Neanderthal skull, an altogether more interesting specimen, and to which he devoted much greater space.
If the reasons for preservation were better understood, DNA experts would know which precious museum specimens were worth sampling and which to leave alone.
The two distinctly annotated specimens in cluster 16, H. "bulliardii" it20_4_1 and H. "olivaceus" zb2804 (an immature specimen) most likely represent identification artefacts (compare cluster 2, which contains most H. bulliardii specimens, and cluster 17, which comprises most H. olivaceus collections).
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CEO of Professional Science Editing for Scientists @ prosciediting.com