Sentence examples for specimens and primary from inspiring English sources

An interesting result was the difference in transcriptional profiles between original frozen specimens and primary culture of all three WHO grades.

The Proteome Profiler™ Human Phospho-RTK Array Kit provided a platform to assess phosphorylation of 42 different receptor tyrosine kinases in primary and metastatic AGASACA tissue specimens and primary thyroid carcinoma samples using the available flash frozen tumor specimens.

It is thus necessary to analyze the pathogenetic mode of the secondary onset of carcinomas in benign pleomorphic adenomas further in vitro, because most of the previous investigations have been limited to only surgical specimens and primary cultures.

HEMs and HEKs were incubated under the indicated conditions were lysed and total protein was extracted from the tissue specimens and primary cells by the RIPA buffer, separated by 10% SDS-PAGE, and electro-transferred onto a polyvinylidene difluoride membrane (Millipore, Billerica, MA, USA).

Cells were lysed and total RNA was extracted from the tissue specimens and primary cells by the SV Total RNA Isolation System (Promega, Madison, WI, USA), and used to generate cDNA with the PrimeScript RT Reagent Kit (Takara, Ōtsu, Shiga, Japan).

It was necessary to increase the distance between neck dissection specimen and primary injection site of the tracer substance to reduce the influence of radioactive scattering on identification of the SN with the gamma probe.

Specimens (slides) and primary antibodies were incubated for 1 hour.

In the 67 patients with both the primary tumor and lymph node metastatic tumor specimens, GCRG213p was positive in 51 lymph node metastasis specimens and 52 primary tumor specimens (76.12% vs 77.61%), respectively, which indicates that elevated GCRG213p expression in lymph node metastatic tumor is concordant with GCRG213p expression in primary gastric carcinoma.

The experimental programme consisted of testing 6 control specimens, and 36 primary specimens initially distressed to 80 85% of theoretical stress level, repairing, and retrofitting prior to testing.

Our efforts are unique in that we utilize clinical MPE specimens, and establish primary culture in an autologous culture TME.

Somatic hotspot mutation analysis for KRAS, PIK3CA, BRAF and EGFR can reliably be performed using allele-specific qPCR with hydrolysis probes; the mutation results from cytological specimens and the primary tumors are highly concordant.