The cytb topology reflected the geographic origin of the specimens, and the five main evolutionary lineages of Fink et al. [33] and Heckel et al. [34] were found: W (France, Germany, Switzerland, Belgium, Spain), F (Germany), I (Switzerland, Italy), E (Germany, Austria, Hungary, Slovakia, Czech Republic, Poland, Ukraine, Russia, Finland) and C (France, Germany, Switzerland, Netherlands, Denmark).
The joints of these specimens were typical in geometry and main reinforcement detailing to specimens of a prototype building frame designed according to ACI318-08.
Present manuscript is a review of the main specimens and equipment where research can be carried out for performing multiaxial fatigue tests under bi-axial stress states.
Two shallow water sequences obtained from GenBank, Oncholaimus spp. and Viscosia sp., appeared more closely related to the divergent Oncholaimus group containing deep-sea specimens, and not to the main clades representing specimens from intertidal sediments.
Nuclear presence of the proliferation marker pKi67 was detected in 10 50% of cells in the PMP-1 primary tumor and main surgical specimen, and this feature was conserved throughout the animal passages.
In the PMP-2 model, low and intermediate Ki67 positive cell fractions were found in the primary tumor and main surgical specimen, respectively, whereas in tissue harvested from the animals the positive fraction was consistently high (>50%).
None of the markers showed significant differences between samples A and B. Similarly, Ki67, ER, PgR and HER2 did not differ significantly between core-cuts and main resection specimen, although there was a trend for lower resection values for ER (P = 0.06).
In the PMP-2 model, the pKi67 expression was less consistent, with low and intermediate Ki67 positive cell fractions in the primary tumor and main surgical specimen, respectively, whereas positive fraction in tumor tissue from the animals was consistently high (>50%).
The two intestinal tumor markers, CEA and CK20, were highly expressed in both primary tumors, main surgical specimens and in all specimens harvested from the first six animal passages, with membranous and cytoplasmic staining observed in 100% of tumor cells in all but one section.
The strains of specimens and the evolution of the main properties with the number of cycles are assessed.
