For cellular specimens grown on grids, grids were removed from the cell culture dish and 2 μl of a colloidal gold suspension (coated with BSA, 10 nm diameter, in HBSS buffer) were added immediately before removing excess of liquid by blotting the grid with a filter paper.
The gold (Au) and silica (Si) peaks in the spectra in Fig. 5 originated from gold sputtering of the specimen and the silica grid used for support of the specimen respectively.
This was followed by herbarium specimens (1073 grid-cells and 11 occurrences ±1 per grid-cell) and, last, photographs (156 grid-cells and 8 occurrences ±1 per grid-cell).
All authors dissected specimens and analyzed images.
Firstly, the generation methods of the random polyhedron aggregate and ITZ are introduced, as well as the process how to create the meso-scale finite element (FE) model of the concrete specimen through grid mapping method.
Controls consisted of specimens on grids treated in the same manner, except that primary antibodies were omitted.
These include identifying suitable areas of vitreous ice at low magnification, determining the presence and location of specimen on the grid, automatically adjusting imaging parameters (focus, astigmatism) under low-dose conditions, and acquiring images at high magnification to either film or a digital camera.
The EV suspension was placed on the gold-loaded, epoxy-embedded specimen grids and in 1% glutaraldehyde, postfixated in 1% OsO4 and stained with 1% aqueous uranyl acetate.
Diluted suspensions and nanoemulsions were deposited on specimen grid (Formvar-Carbon support films, Electron Microscopy Sciences), negatively stained with uranyl acetate solution (2% w/v) [37] and observed at different magnifications.
About 3 μl of aliquots (∼20 μg protein/ml in 50 mmol/L Tris, pH 7.6, 100 mmol/L ammonium sulfate, 10% (v/v) glycerol, 1 mmol/L EDTA, 10 μmol/L ZnSO4, 0.02% NP-40, 10 mmol/β-ME) was applied to a carbon-coated 400-mesh Cu EM specimen grid freshly glow discharged and was then preserved by staining with 0.75 % (w/w) uranyl formate solution.
