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This prompted us to use clinical specimens and functional studies to investigate the pro-tumourigenic and pro-metastatic function of INH α.
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We attempted to identify novel biomarkers and therapeutic targets for esophageal squamous cell carcinoma by gene expression profiling of frozen esophageal squamous carcinoma specimens and examined the functional relevance of a newly discovered marker gene, WDR66.
In a first stage, we used the Olympus® 2.5 mm forceps (standard-oval without needle; required work channel size: 2.8 mm Ø; Shinjuku, Tokyo, Japan) and in a second stage the Endoflex® 3.4 mm (jumbo-oval without needle; required work channel size: 3.7 mm Ø; Voerde, Germany) in order to obtain larger specimens, optimizing the adequacy of the specimens for functional and biochemical studies.
Specimens were considered adult when they had fully erupted and functional dentition as well as closed or fused spheno-occipital and/or spheno-ethmoid sutures.
P-gp phenotype can be detected in clinical specimens through methods such as reverse transcription-polymerase chain reaction, immunodetection, and functional assays employing fluorophore substrates.
By immunohistochemical staining of renal biopsy specimens, it has been shown that urinary LFABP (U-LFABP) excretion is highly associated with structural and functional tubular kidney damage.
By studying renal biopsy specimens with immunohistochemical staining, it has been shown that u-LFABP excretion is closely associated with structural and functional tubular kidney damage (13).
In this study, we aimed to examine BRMS1 expression and functional status in vitro and in vivo and to explore its clinical significance in clinical NPC specimens.
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