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Using procedures developed by the Rosenberg group, we have analyzed TILs from 40 melanoma specimens and established standard operating procedures for generating TILs for therapeutic use.
We first examined the expression of Hh components in GBC specimens and established the expression of Gli1, Shh, and Smo.
Standard cytogenetic and immunofluorescence analysis was performed on pre-treatment bone marrow, peripheral blood specimens and established cell lines.
In contrast to normal brain tissue, primary tumor specimens and established medulloblastoma cell lines overexpress both TAp73 and ΔNp73 proteins, consistent with dysregulation of developmental expression and a possible contribution to their neoplastic phenotype.
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Our efforts are unique in that we utilize clinical MPE specimens, and establish primary culture in an autologous culture TME.
A reference specimen is computed and established as the mean of the fitted landmark coordinates.
Using the ALDEFUOR assay, ALDH1 activity was also examined in 16 primary tumor specimens and 43 established human epithelial cancer cell lines.
Using the ALDEFUOR assay, ALDH1 activity was also examined in 16 primary tumor specimens and 43 established epithelial cancer cell lines.
To develop a standard operating procedure (SOP) for collection, transport, storage of human endometrial tissue and blood samples, subject and specimen annotation, and establishing sample priorities.
To address these problems, we used validated ER β antibodies [ 58, 67] and established TNBC specimens (ER α-negative/PR-negative/HER2-overexpression-negative).
These approaches, however, relied on organ preparation by dissection of the specimens and the establishing of amino acid sequence information by tandem mass spectrometry for construction of a cladogram.
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