Implant sites were allowed to heal for 2 weeks, after which specimens were retrieved for morphometric assessment by using backscatter scanning electron microscopy and the Bioquant Image Analyzer.
The side and bottom surface were coated with epoxy, except top surface for the test after which specimens were immersed in 10% concentration of NaCl solution for 91 days.
The specimens were then fixed with 70% methanol for two weeks, with a fresh solution change after one week, after which the specimens were dehydrated in an ascending series of ethanol (70, 96, and 100%), changing the solutions twice weekly.
Next, 0.5 mL of TBA was added to the supernatant; after which, the specimens were incubated in a water bath at 100°C for 10 minutes.
Moreover, the quantitative fractography analysis were conducted over the ruptured specimens after tensile tests, which confirms the influence of element boron on eradicating the coarse intergranular carbides.
Another study conducted by Moser et al. (2009) showed a maximum ASR expansion of 0.02 % for both undamaged and pre-damaged UHPC specimens after 600 days, which is lower than the threshold limit of 0.04%% (Moser et al. 2009).
The sample was first loaded and unloaded in the elastic regime only at the low strain rate, after which the specimen was immediately reloaded with a high strain rate of 1.8 s−1.8
The dates of Dr. Hedges's specimens and, more telling, the dates after which the lizards ceased to be collected, correspond almost exactly with the introduction of the mongoose.
The pre-compression stresses were applied after 4 h, 8 h and 24 h of accelerated curing at 40 °C, after which the conditioned specimens were allowed to continue curing under constant conditions.
Blood specimens were collected from each participant, after which PBMC were isolated, and protein extracts prepared and assayed for APE1 enzymatic activity under the optimized conditions described above.
