The plasmids were spiked into individual replicates of each of the ten specimens after extraction; one replicate of each of the 10 specimens was not spiked with plasmid and was used as the control.
Tensile coupon tests were performed on specimens after exposure to one of 13 preselected maximum temperatures (up to 1000 °C).
The area of the fluorescent lens capsule epithelium specimens was determined after one and two weeks of culture using image processing software (ImageJ, National Institutes of Health, USA) and the increase in cell layer surface was calculated.
Mass-specific respiratory (ṀO2) and photosynthetic (Ṗg) rates of each specimen were measured after one week in normocapnic acclimation conditions and after the 6-week experimental period.
After one month, specimens were harvested, resin embedded, serial sectioned and examined under fluorescent light microscopy.
Either analysis of serum specimens of patients one year after treatment shows promising results in the meaning of shift to the population of healthy individuals.
After one day, the specimen were demolded and retained in the curing room where the relative humidity and temperature were maintained at 95%and2525 °C, respectively.
The specimens were then fixed with 70% methanol for two weeks, with a fresh solution change after one week, after which the specimens were dehydrated in an ascending series of ethanol (70, 96, and 100%), changing the solutions twice weekly.
MMP-I activity of the MDSC/SIS was significantly higher (p<0.0025) after one day in culture compared to specimens collected from subsequent time points and the unseeded control.
One work group reported possible identification of serosa or subserosa on gastrectomy specimens after fixation with formalin [ 33].
Cube specimens measuring 50 mm × 50 mm × 50 mm were prepared from POFA-EACC and exposed to the sulfate solutions after one day of heat curing.
