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All patients, from whom a clinical specimen yielded a KP or EC isolate resistant to Colistin (VITEK2 COMPACT,bioMerieux, Hazelwood, MO).
If a specimen yielded a positive H5 band, the specimens were confirmed by different RT-PCR primers and by real-time RT-PCR (12 ).
A broncho-alveolar lavage specimen yielded a mixed culture of multidrug-resistant M. bolletii and Klebsiella pneumoniae despite negative results of direct examination.
The other positive specimen, termed PML/2011, was from an adult female Neoromicia cf. zuluensis bat sampled in 2011; the specimen yielded a novel betacoronavirus (GenBank accession no. KC869678).
A fine-needle aspiration specimen yielded a gram-negative rod on fungal media (IMA agar) after 15 days of incubation; 16S rDNA sequencing confirmed that the isolate was G. bethesdensis.
Attempted PCR amplification of a 630-nt portion of the rickettsial ompA gene (nt 70 to 701) (4) from the early serum specimen yielded a product of the expected size.
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Very large female Murray cod can have egg counts as high as 80,000 90,000, although a recent, very large 33 kg specimen yielded an egg count of 110,000 viable eggs.
As a result, the performance of both smear techniques on the same specimen yielded an absolute increase of 12 13% in sensitivity over either smear alone (p < 0.01 for increase in sensitivity over direct or concentrated smear alone).
All specimens yielded a yellow pigmented rapidly growing mycobacterium species.
New left lung nodules were biopsied and specimens yielded a mold morphologically identified as A. fumigatus.
Two of these specimens yielded a sequence type consistent with the template Z83958; the remaining nine specimens yielded the new type.
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CEO of Professional Science Editing for Scientists @ prosciediting.com