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The Illumina reads for each specimen were screened using the FASTX-Toolkit [ 19] to remove the first 15 and last 6 nts.
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Subsequently, the specimen was screened by a single-nucleotide polymorphism assay distributed by Canada's National Microbiology Laboratory and the World Health Organization pyrosequencing protocol for the presence of the H275Y mutation (8 ).
All specimens were screened for anti-B19V NS1 IgG and IgM.
DNA specimens were screened using two nested PCR assays.
Clinical specimens were screened for influenza A virus by multiplex real-time PCR and then subtyped as H1 or H3 with specific primers.
Mosquito specimens were screened for knockdown resistance (kdr) and insensitive acetylcholinesterase (ace-1R) mutations using AS-PCR and PCR-RFLP, respectively.
Subsequently, living specimens were screened under a dissecting microscope, and nematodes with different morphological features and/or different behavior were handpicked, temporarily mounted in a microcompressor slide (Taylor's Microcompressor Mk II; Taylor, 1991) and heat killed.
In total, 102 specimens were screened, yielding 41 species belonging to 33 genera (Table S1, 1 41), representing all families, subordos or ordos involving marine taxa as indicated by Meldal et al. [14], except for the subordo Desmoscolecida, of which we had no specimens.
In addition to the EDII fusion peptide and the EDIII cross-reactive epitope knock-out antigens, serum specimens were screened with an EDII−EDIII knock-out antigen, combining the same substitutions from the individual mutant antigens in these two antigenic regions (EDIIFP−EDIIICR; Table 2).
All specimens were screened for multiple serotypes.
Serum specimens were screened at a dilution of 1 32.
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