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Trypsin-digested, fractionated peptides from each clinical specimen were independently identified and quantified using a MudPIT approach using HPLC-Chip nano ESI-MS/MS analysis.
The total number of spectra from either benign or PDAC specimen were independently normalized, and the normalized cumulative spectral counts of all the peptides for each protein were used as a measure of protein abundance.
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Individual specimens were independently analysed by two of us (M.P and F.S), without knowledge of mice genotype.
The histopathologic characteristics of all tumor specimens were independently reviewed by experienced hematopathologists.
The immunostained specimens were independently evaluated by two blinded investigators (HB and HO).
In each case, histopathologic specimens were independently reviewed by two pathologists.
The specimens were independently examined by an experienced pathologist (SI) and a resident (OB).
The histological specimens were independently assessed by two gastroenterologists with no knowledge of the clinical findings of the patients.
Seven bone specimens were independently extracted, amplified, cloned and sequenced in the ancient DNA laboratory of the Centre of Alpine Ecology (Edmund Mach Fundation, Trento).
A total of 40 specimens were independently estimated for TSP by two observers (FJAG and JE) blinded to the patient outcome and the other observer's score.
This relationship is best depicted after the 18 tumor specimens were independently divided into three groups each containing 6 tumors based on c-Met expression levels (highest, middle, and lowest thirds) and similarly into three groups based on DR5 expression levels.
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