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The ThinPrep and touch prep slides of each specimen were fixed in Carnoy's fixative (3:1, methanol: acetic acid) according to standard procedures and submitted to the series of hybridizations using an optimized protocol.
For SEM, specimen were fixed in Sodium-Cacodylate buffer, pH 7.3 (Merck, Darmstadt, Germany) containing 2.5% Glutaraldehyde (Polysciences, Warrington, P.A., U.S.A) for 24 hours, dehydrated by insertion in increasing acetone dilutions and dried with a CPD030 (Bal-Tec, Balzers, Liechtenstein), followed by gold sputtering with a SEM Coating System (Polaron, East Grinstead, United Kingdom).
The specimen were fixed in a special conic embedding system with cement.
The biopsy specimen were fixed in formalin and processed as standard for haematoxylin and eosin staining.
The specimen were fixed in 10%% phosphate buffered formaldehyde and embedded in paraffin.
The specimen were fixed in 4% buffered formalin and embedded in paraffin.
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The specimen was fixed in 10% formalin, and paraffin sections were prepared.
One antral specimen was fixed in formol solution at 10% and embedded in paraffin.
Each specimen was fixed in 10% formaldehyde and embedded in paraffin.
Each kidney specimen was fixed in 4 % paraformaldehyde (PFA) for 24 h and paraffin embedded.
Kidney specimens were fixed in 0.1 mol/L phosphate-buffered Karnovsky's fixative.
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