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Four drops of the specimen were applied to the test and the result was read after five minutes.
Ten, 5 and 3 microlitres of each specimen were applied by a microlitre syringe (N 701, Hamilton, Bonaduz, Switzerland) in 1 centimetre distance to the bottom of the plate.
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So far, cryo-FIB milling of frozen-hydrated specimen was applied without direct ultrastructure imaging as a preparatory step for thinning samples to a suitable size for cryo-transmission electron tomography, instead of cryo-sectioning (Marko et al. 2006; Hayles et al. 2010; Rigort et al. 2010, 2012; Wang et al. 2012).
Axial loads and bending moments about the longitudinal axis of the specimens were applied.
For the investigations, fine polished tubular specimens were applied which were analysed for microcracks on the specimen's surface after a defined number of cycles.
The flattened Brazilian disc specimens were applied to determine the tensile properties and energy absorption capabilities under high strain rate by an SHPB (Split Hopkinson Pressure Bar) and medium strain rate by a drop-hammer testing machine.
Purified virus specimens were applied to carbon-coated grids and were stained with 1% (w/v) potassium phosphotungstate (pH 6.5).
Bat serum specimens were applied at dilutions of 1 500 and 1 2,000.
The serum specimens were applied to the spot slides at dilutions of 1 40 for IgM detection and 1 80 for IgG detection.
In the first, the same primers used in the investigation of the ancient DNA specimens were applied to samples from two modern muskoxen (both hair and blood from the first, blood only from the second, unrelated individual).
The proportion of pH1N1 positive specimens was applied to estimates of ILI, hospitalizations, and deaths to estimate disease burden.
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