From prior review of these normal specimens [ 22], we had data on the number of lobules within each specimen, and we selected samples with an adequate number of lobules to evaluate (8 or more).
However, the specimens that we selected from both lineages cover a large part of their respective geographical distributions, including samples from the edges of their ranges.
Beginning with the largest specimens from each event, we selected 10 specimens per known parasitoid morphospecies and 5 specimens per other morphospecies for molecular analysis.
We selected specimens expressing mem-TdTomato in single neurons for detailed analysis by confocal microscopy to find single fibers in 100% of the cases (N = 42) (Figure 2 D), indicating that neurons do not project branched fibers towards any individual neuromast.
Among the specimens from these participants, we selected "cases" who had high CD8+ T cell activation and low absolute CD4+ T cell counts at the time specimens tested were obtained, and were defined as: Percentage of circulating CD8+ T cells co-expressing CD38 and HLA-DR was >10%.
It is important to note that because our study was a countrywide assessment, we selected specimens for analysis without regard to expected exposure.
In L. granatensis, we selected specimens harboring either L. granatensis or L. timidus mtDNA based on the polymerase chain reaction (PCR -restriction fragment length PCR -restrictionLP) assay described by Melo-fragment et alength05).
We selected retrospectively from our files, 222 cases as follows: 166 specimens were BM of gastric type (GTBM) that underwent follow up; moreover, we selected 37 specimens of Dy and 19 of Ca that did not undergo follow-up.
Of 39 stool specimens that were found to contain G12 rotavirus by the typing reaction with our G12 primer pair, we selected 12 specimens from those samples that produced the full-length VP7 amplification product for nucleotide sequencing.
For each case, we selected two specimens from the DoDSR, one collected ±7 days of the case diagnosis (acute), and the first specimen collected >3 weeks before their acute specimen to account for the maximum typical incubation period of chlamydia and gonorrhoea (Hook and Handsfield, 2008; Stamm, 2008; pre-acute, range: 22 dayearsyears before diagnosis).
