After the extraction of the first 45 laryngeal specimens, we decided to systematically carry out WGA on all extracted DNA.
Notwithstanding some Authors showed that aerosol-induced sputum technique was more effective for detecting ferruginous bodies than spontaneous sputum specimens [ 19], we decided to test the latter one method because it was more acceptable, considering the local social context.
As limited attention has been given to metastatic melanoma clinical specimens yet [ 7, 8], we decided to analyse a representative number of resection specimens for the presence of mutations in the activation segment (exon 15) of the B-raf kinase domain in cutaneous/subcutaneous melanoma metastases.
Based upon these observations, we decided to collect specimens at 6 and 12 weeks for mechanical testing and 3 and 6 weeks for histological analysis.
We decided to include this specimen in our analysis despite the preliminary state of its identification because of its relevance to the aim of this work.
For that reason, we decided to collect most of our clinical specimens by fine-needle aspiration biopsy (FNAB) taken from consecutive suspicious breast tumors at the time of clinical diagnostic core biopsy.
We decided to study renal cell carcinoma as example for clinical specimens, since our group have already investigated in previous studies [ 4] the expression of the two FABP-types B-FABP and L-FABP in renal cell carcinomas.
We decided to look for alternative solutions that would achieve tomographic reconstructions of living specimens in a high-resolution, high-NA SPIM setup without any modifications to the hardware.
In order to better define the possible prognostic value of MMP14 expression in MM, we decided to investigate MMP14 expression by immunohistochemistry in a group of well-characterized MM specimens.
Since all molecular methods tended to split morphospecies, we decided to risk taxonomic lumping, and classified ELs as the most comprehensive grouping of specimens predicted by any of the five delimitation methods.
