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Each specimen was separately attached to filter paper and fixed immediately.
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Human specimens were separately analyzed with respect to progenitor phenotypes to reveal individual to individual variability.
The specimens were separately stained with hematoxylin and eosin (H&E), periodic acid Schiff stain, and Alcian blue stain.
Isolated chondrocytes from individual specimens were separately cultured with DMEM/F-12 plus 10% FBS at 37°C under a humidified 5% CO2 atmosphere until reaching confluence.
Isolated chondrocytes from individual specimens were separately cultured with DMEM/F-12 (GIBCO, Life Technologies, Paisley, UK) plus 5 % FBS (Invitrogen, Life Technologies, Paisley, UK) at 37 °C under a humidified 5%% CO2 atmosphere until reaching confluence for 4 6 days.
Isolated chondrocytes from individual specimens were separately cultured with Dulbecco Modified Eagle Medium/Ham F-12 (DMEM/F-12) (GIBCO BRL, Paisley, UK) plus 5% fetal bovine serum (FBS; Invitrogen, Life Technologies, Paisley, UK) at 37οC under a humidified 5% CO2 atmosphere until reaching confluence for 4 to 6 days.
When multiple specimens were received from a single patient, each specimen was processed separately.
Frequency of methylated target sites in each type of tissue specimen was calculated separately for each gene using the probe-specific threshold values.
A brain specimen from each mouse was separately fixed in 10% neutral buffered formalin for at least 24 h and embedded in paraffin for pathological studies and immunohistochemical detection of GFAP.
was separately measured.
Her identity was separately verified.
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CEO of Professional Science Editing for Scientists @ prosciediting.com