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The C. cornuta specimen was provided by the Peabody Museum of Natural History with the help of Greg Watkins-Colwell.
A broad habitus view of the entire specimen was provided by combining separate images into a composite form.
A single tissue specimen was provided for 27 patients, and multiple specimens (2 3, collected from different anatomic sites or at different times) were provided for five patients.
The histological diagnosis of each specimen was provided by standard light microscopic evaluation of the routinely processed and parafin embedded tissues.
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By means of illustration, a typical DIC movie showing the loading and the unloading phase of the specimen is provided in the Additional files 1 and 2. To overcome the practical issues with the analysis of the data due to the local heterogeneity of the different strain maps, a cumulative strain distribution was created.
The oyster ID, geographic origin and collection date of each specimen are provided in Table 4.
A histology specimen is provided in panel G that shows the collapsed dorsal vein and the spinal cord gray matter and white matter.
Additional information about these specimens was provided previously.[28] Total RNA was isolated as described above and cDNA was synthesized using MMLV-reverse transcriptase kit and Oligo dT 12 18 primer (Invitrogen), according to manufacturer's protocol.
Initial evidence of WNV in the specimens was provided by nested PCR amplicons co-migrating with the WNV positive control.
Support for the collection of research specimens was provided by the National Oceanographic and Atmospheric Administration (NOAA; United States), NOAA's National Undersea Research Program, and UNCW's Coastal Oceans Research and Monitoring Program.
Among the 172 patients who underwent standard treatment, informed consent to use specimens was provided from 167 patients, for whom the median follow up term was 55 months (range 11 122 months).
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