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To avoid any eccentricity, each column specimen was adjusted in such a way that the center line of axial loading coincides with the longitudinal axis of the column, and to ensure the complete contact with the loading plates, the top and bottom end surfaces of each column specimen were leveled with a layer of high-strength plaster powder.
For selective capturing and expression-profile analysis of anionic protein species from CSF, 2 µg of total protein from individual specimen was adjusted to a final volume of 200 µl with binding buffer [20 µM Trisma base (pH 8) containing 0.1% Triton-X 100].
The angle of the specimen was adjusted to make the testing device, screw adjuster, and the direction of screw insertion lie on a straight line.
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In the second phase, water cementitious binder ratios of the specimens were adjusted to attain a constant unconfined concrete strength for specimens containing different amounts of silica fume.
Intensity levels of digital images in experimental and control specimens were adjusted proportionally and in the linear range either in Axiovison or Photoshop CS2 (Adobe).
Urine specimens were adjusted to pH 3.0 and stored at −20°C until extraction and analysis.
Urine specimens were adjusted to pH 3.0, aliquoted into test tubes, and stored at −20°C until extraction and analysis.
The lateral position of the specimen chamber was adjusted by micrometer screws (BM11.16, Newport, Darmstadt, Germany), and axially by a motorized stage (M105.1B translation stage with DC-Mike linear actuator M232-17 from PI, Karlsruhe, Germany).
The specimen height was adjusted to the point of initial contact between the indenter and cartilage surface, and samples were positioned as described previously (Han et al. 2010, 2012; Madden et al. 2013).
Dry soil passing 2 mm sieving was used to make a cylindrical specimen, and the dry density was adjusted to the same value as the in-situ dry density of the soil.
Data were collected using a Philips CM-100 microscope fitted with a 1K×1K Gatan Multiscan 794 CCD camera with magnification set at ×61000, which corresponds to 3.93 Å per pixel at the specimen level; the underfocus value was adjusted to ∼1.0 μm.
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