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This covers preparation and description of the specimen used the targeted gene, detection reagent type, probe sequences and cell line data.
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Total RNA was obtained from tissue specimen using the Trizol reagent (Gibco BRL, MD) [9], [44].
Fluorescence excitation was achieved using a 25 mW Argon ion laser (Spectraphysics, Mountain View, CA) and illuminating the specimen using the 488 nm and 514 nm lines.
The coordinates of 10 landmarks were registered for each specimen, using the software tpsDIG 2.10 [ 36].
Total genomic DNA was extracted from the muscle of a specimen using the OMEGA Genomic DNA Extraction Kit.
Urinalysis was performed on aliquots of the MSU specimen using the QuickVue UrinChek™10+SG test strips.
Nucleic acids and protein fraction are isolated from tumor specimen using the AllPrep method and automated using QIAcube machines (Qiagen).
The aim of performing FNA is to obtain a diagnostic specimen using the fewest number of passes possible.
Genomic DNA was extracted from each individual specimen, using the DNeasy Blood & Tissue Kit Qiagen Sciences Inc.., Germantown, MD), following the animal tissue protocol.
Total genomic DNA was extracted from the legs of one adult for each specimen using the Qiagen™ DNAeasy kit.
Fibrosis staging of each surgical resection specimen using the modified Ishak method was performed by a single liver pathologist.
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