In contrast, the routine use of viral multiplex real-time PCR (mRT-PCR) assays allows a single respiratory specimen to be screened for a large number of respiratory viruses easily within the working day [4].
In the event of a screen-positive result, most programs rely on information submitted with the newborn screening specimen to identify the newborn's physician or medical home.
A nested real-time reverse transcription PCR (RT-PCR) specific for the WNV NS5 gene that distinguishes between lineages 1 and 2 with WNV-specific FRET probes on the basis of dissociation curve analysis was used to screen specimens (23 ).
To test this hypothesis we used IS2404 qPCR to screen faecal specimens from confirmed BU patients, household contacts, and control samples from BU non-endemic regions in Ghana for the presence of M. ulcerans DNA.
Primers used to screen respiratory specimens were identical to those described by Woo et al. (14 ).
In order to obtain an exon specific, cost effective and high throughput method to screen ESCC specimens for FHIT and WWOX genomic deletions, a new assay was designed using the existing multiplex ligation-dependent probe amplification MLPA technology [ 33].
We envision its use as a diagnostic tool, amenable to high-throughput application, to screen patient specimens (e.g., serum, urine, pleural fluid, and cerebrospinal fluids for tuberculous meningitis, etc).
Seven met our stringent criteria for possible methylation-sensitive miRNAs and were used to screen patient specimens (205 CRCs and 36 ECs) by methylation-specific multiplex ligation-dependent probe amplification.
In brief, private laboratories across Germany agreed to screen stool specimens of gastroenteritis patients for the presence of Shiga toxin 1 and Shiga toxin 2 with an enzyme immunoassay if predefined criteria were met (e.g., patients with diarrhea were <5 years of age, bloody diarrhea was mentioned on the laboratory request form).
