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Based on previous reports and recommendations, tissue collected from the eschar site is the most likely specimen to provide a positive result for rickettsia PCR or culture (11, 12 ).
We used a 40x 0.8 NA water immersion objective (for its long working distance), and placed a coverslip on top of the intact specimen to provide a barrier between the oil/glycerol mixture and the immersion water column (though in principal, an objective designed for oil/glycerol immersion would improve our results).
For guidance during needle insertion, positioning, and biopsy, and to acquire a 3-D OCT image of the biopsied specimen to provide a microscopic "first look" of the specimen prior to sending for histopathological sectioning and analysis, the OCT catheter is inserted into the needle-biopsy hand-piece which has been modified to include optical windows at specific locations.
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Aside from presenting an approach for using pre-1900 muspecimensimens to provide a baseline for historic diversity, the study effectively yanks the rug out from under cutthroat trout restoration efforts and raises the stakes in a lawsuit filed last week by the Center for Biological Diversity against federal land managers.
Here, we describe newly developed classroom exercises that make use of emerging biodiversity informatics resources—"big data"—obtained from museum specimens to provide authentic, inquiry-driven, place-based instruction in the scientific principles and procedures underlying modern climate change research.
Virus culture should be performed on at least a sample of specimens to provide material for antigenic determination and potential isolates for vaccine production.
This is not to imply that every State should store all specimens forever but, rather, that there should be a sufficient number of States with diverse populations and long-term storage of residual specimens to provide this critical resource.
The extraction of eigenvectors for such VCV preferably requires ca. 30 specimens to provide reliable estimates [ 47, 48]; the robustness of the estimate nevertheless depends on the structure of the data [ 20].
Twenty nine specimens failed to provide a sequence for both plastid markers, creating 871 specimens with sequence records for both ITS2 and one of these genes (rbcL & matK from 68%, rbcL & ITS2 from 82%).
It was only then that the Italian authorities were in a position "to penalize trade in, or possession of, such specimens," and "to provide the confiscation or return to the State of export of such specimens".
If the 62000 skull showed that 1470 was not a single odd individual, the other two specimens seemed to provide a vital piece of evidence that had been missing.
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