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While LCM has the advantage of preserving spatial relationships within a tissue specimen, the tissue must be sectioned, often at thicknesses smaller than the diameter of single cell, leading to loss of chromosomal material [8].
After the collection of the colorectal cancer specimen, the tissue was minced with a blade into pieces smaller than 2 mm cube and digested overnight with triple enzyme buffer containing collagenase IV (1 mg ml−1, Sigma, no. C-5138), hyaluronidase (100 μg ml−1, Sigma, no. H-6254) and deoxyribonuclease (20 U ml−1, Sigma, no. D-5025) in RPMI1640 medium.
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We thank Katherine Deschryver, MD from Washington University School of Medicine for her histological review of tissue specimens for the tissue reproducibility study.
There were DCNAs shared by the cell lines and tumor tissue specimens and recurrent DCNAs in the tissue specimens were generally frequent in the cell lines.
Specimens were from the tissue bank at the Silesian Center for Heart Disease (Zabrze, Poland).
Cutting or core biopsy needles provide specimens that preserve the tissue architecture enabling histological analysis.
performed quantitative analysis of the tissue specimen without knowledge of specimen identification.
RNA was extracted for fresh tissue specimens (from 10 colon carcinoma specimens and 10 adjacent normal colon tissues) with identifiable tumor in the tissue specimen.
For isolation of DNA from paraffin-embedded tissue specimens the EZ1 DNA tissue kit (Qiagen) procedure was slightly modified.
Formalin fixation followed by paraffin embedding of tissue specimens is a widely used preservation method because it helps to maintain morphological features of the tissue specimen [ 34].
Therewith, the structure of the MGU closely resembles the shape of the tissue specimen after a Swift operation.
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