These preliminary data provided the rationale to embark on a large-scale sequencing project, and the sequences necessary to construct probes for HoxA1, HoxA13, and HoxD11 that subsequently were used to screen an arrayed 10X coverage bacterial artificial chromosome (BAC) genomic library from a single paddlefish specimen that was constructed at the Benaroya Research Institute (Seattle, WA).
A hiker from Vancouver, British Columbia, wrapped his arms around a small Blue Lake specimen that was bent nearly double.
Then he removed the original specimen that was the subject of the Nature paper.
"That would have been a perfect tooth," he said, smiling and holding up a large specimen that was sheared in half.
Their tumors were small, they had wide margins (the space between the outermost precancerous cells and the edge of the specimen that was surgically removed), and they were of a low-grade, non-comedo type.
Most of these seem to represent Prionosuchus, but one little specimen that was found by Domingas da Conceiçáo, another of Juan's undergraduates, seems to represent a new kind of amphibian.
The specimen that was strengthened with X-shaped CFRP strips was failed with premature shear failure.
Another specimen that was morphologically identified as G. "filamentosa" occupies the basal lineage of the G. pacifica assemblage (Figure 1).
But no one in Latin America had found a wild-living specimen that was carrying the virus.
The specimen that was immersed in 0.2 % APF solution fractured near the critical stress for martensitic transformation without martensitic transformation.
The molecular methodology gave 8 positive tests from the same set of biopsies, including from the 1 specimen that was determined to be positive microbiologically.
