Information for collection location, climate group and year, specimen specific ID number, sex, result for wsp and 16S rDNA screening and GenBank accession number of COI partial gene are included.
We thank H. Frahm for useful comments, advice and making specimen specific data from Stephan et al. [37] available.
36 On the basis of the immunostaining patterns in cytology, p27 positivity alone with no Galecin-3 and HBME-1 stain is (100%) diagnostic of benign lesions, and Galectin-3 and HBME-1 dual positivity (Galectin-3-positive cells >30%, and HBME-1-positive cells >10% in 1 specimen) is 100% specific for malignancy.
Using parametric computational analysis of high-resolution micro-CT-based finite-element models of cores of elderly human vertebral trabecular bone, a specimen-specific correction factor for the side-artifact was quantified as the ratio of the side-artifact-free apparent modulus (Etrue) to the apparent modulus that would be measured in a typical experiment (Emeasured).
To identify the enterovirus type in the specimens, RT-PCR, specific for a partial sequence of the viral protein 1 (VP1) region, was performed by using the COnsensus-DEgenerate Hybrid Oligonucleotide (CODEHOP) Primers (bioinformatics.weizmann.ac.il/blocks/codehop.html) (5 ).
Though TA is thought to play an important role in the development of most cancers [ 5], its detection in testicular tissue specimens is not specific for malignant disease [ 6, 19].
HCII LR HPV positive specimens (probe A specific for 5 LR types: 6,11,42,43,44) were typed using HPV 6 and HPV 11 specific primers and resulting negative samples were considered positive for the other LR types included in probemix A (HPV 42,43,44).
Specimens for dynamic melt rheology were produced by ultrasonicating the nanoparticles (at a specimen-specific concentration relative to the copolymer) for 30 min in toluene to achieve a satisfactory dispersion, followed by copolymer dissolution and further ultrasonication, and then air- and vacuum-drying, all performed at ambient temperature.
To determine whether these cells express MMP-2 or MMP-9, or both gelatinases, we co-immunostained the ISZ specimens with the antibodies specific for the respective proteins.
Neonatal pups were genotyped by PCR analysis of genomic DNA extracted from tail-biopsy specimens, by using primers specific for the β-galactosidase reporter gene (5'-CGGATAAACGGAACTGGAAA-3' and 5'-TAATCACGACTCGCTGTATC-3') (Sigma-Genosys, Haverhill, UK).
