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Continuous carbon-film grids were glow-discharged (PELCO easiGlow, Redding, CA) at 15 mA for 30 s. Next, 5 µl specimen solution was loaded immediately onto the grid and blotted after 15 s.
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After synthesis and cooling the AgNp and Au Ag nanocomposite solutions to room temperature, 100 µl specimen of each solution was taken in a 96-well plate for UV-visible spectroscopy.
The surface activity of the specimens in Hanks' solution was GD > GA > GB > GC.
For specimen preparation, 5 μl solution was spotted onto freshly glow-discharged carbon-coated electron microscopy grids (Ted Pella, Redding, CA).
The surface oxide layers formed with MAO and chemical treatments on Zr disks were characterized using surface analyses; the calcium phosphate formation on the specimens after immersion in Hanks' solution was evaluated.
The tensile strength of the specimen immersed in 0.2 % APF solution was completely recovered by aging at room temperature for 240 h, whereas that of the cathodically hydrogen-charged specimen was only partially recovered by aging at room temperature for 240 h (Yokoyama et al. 2007).
In addition, the tensile strength of the specimen immersed in 0.2 % APF solution was completely recovered by aging at room temperature for 240 h.
The tensile strength of the specimen immersed in 0.2 % APF solution was completely recovered by aging at room temperature for 240 h, where the dominant factor that influenced the tensile strength of the specimens was probably the hydrogen concentration in the vicinity of the specimen surface.
Specimens were fixed in different solutions (Carnoy's solution was routinely used for samples processed in Milano, while 3.7% formalin solution was routinely used for samples processed in Firenze).
After 4-hour trypsin treatment, the trypsin solution was removed and the PG-degraded specimens were washed with PBS solution for three times.
Since the two contrast mechanisms work in different ways for different specimens and for different parts of the same specimens, the only solution is empirical preliminary tests to find the optimum distance between sample and detector and the corresponding optimum level of staining.
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