Although the pre-crack length of the specimen was set to 50 mm to insert the PTFE sheet, an initial crack front is not sufficiently sharp, and overestimation of the G IC value is expected at the first part of the crack propagation.
The estimated power at the specimen was set to 1 mW/mm, as measured with a photodiode (Thorlabs).
For quantification of protein levels, the relative amount of pAkt (Ser-473), pAkt (Thr-308), and pS6 in tumor tissue specimens was expressed as a ratio of the optical density for the tumor specimen to that for the corresponding non-neoplastic specimen (set at 1.0) by densitometric analysis, as described previously [ 16, 17].
For quantification of these proteins, the relative amount of phosphorylated Akt (Ser-473), Akt, or phosphorylated S6 ribosomal protein (Ser-235/236) in tumor tissue was expressed as a ratio of the optical density of the band for the tumor tissue specimen to that for the corresponding normal tissue specimen (set at 1.0) by densitometric analysis, as described previously [ 15, 20, 21].
For quantification of these proteins, the relative amount of phosphorylated S6 ribosomal protein (Ser-235/236) in tumor tissue was expressed as a ratio of the optical density of the band for the tumor tissue specimen to that for the corresponding normal tissue specimen (set at 1.0) by densitometric analysis, as described previously [ 27, 28].
For comparison of DOCK8 expression intensity in healthy controls, DOCK8 geo MFI values were presented as means ± SD, for comparing DOCK8 expression in healthy and diseased specimen, controls were set to a 100% ± SD.
The specimens' sizes were set to a maximum of 15 × 15 mm, and their heights were limited to 1 mm, which kept their total volume small.
The two signatures allowed patients with specimens in the test set to be divided into four quartiles with widely disparate median lengths of survival (13.6, 11.1, 10.8, and 3.9 years), independently of clinical prognostic variables.
Mechanical behavior and failure modes of a reference set of specimens are compared with the results of two specimen sets exposed to aging.
A universal testing machine was used to apply a shear-type stress on the specimen at the crosshead speed set to 3 mm/min which is preferred commonly in laboratory studies.
