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We designed, constructed, and tested a single-beam optical trapping instrument employing twin electro-optic deflectors (EODs) to steer the trap in the specimen plane.
The spiral aperture design has a major advantage over the previously used forked aperture in that the three beams with topological charge m=−1, 0, and 1 are not side by side in the specimen plane, but rather on top of each other, focussed at different heights.
In those conditions, the sample would mostly shrink with two different compression factors, a larger one (gamma _Z) along the normal axis and a smaller one (gamma _perp) within the specimen plane (if (gamma _perp simeq gamma _X simeq gamma _Y)).
We verified that 50 mW laser power reaching the specimen plane and focused on the growth cone did not affect its motion for at least 1 h.
Fluorescence transmission was passed through a laser grade eGFP filter set (Semrock), and collected by a cooled-CCD (ORCA ER II; Hamamatsu) which has a pixel size of 67 nm×67 nm at the specimen plane.
After leaving the specimen plane, the diffracted light is collected by the MO.
Similar(46)
Application is made to the typical uniaxial tension specimen under plane strain conditions in order to chow the predictive capabilities of the proposed micromorphic model, particularly against its ability to give (at convergence) a mesh independent solution even for high values of the ductile damage (i.e., the macroscopic cracks).
By carefully adjusting the position of the fibre in front of the aspheric lens with a two-axis micromanipulator (part number HPT1, Thorlabs), we projected a sharp image of the illuminated fibre core (spot) onto the specimen focal plane selected by the (infinity corrected) objective lens.
Six different types of tests were performed to fracture, including tensile tests on classical dog-bone specimens, flat specimens with cutouts, plane strain grooved specimens, and punch indentation tests on circular blanks.
The experimental testing campaign includes tension torsion tests under proportional loading, standard tensile tests, tensile tests on notched round and flat specimens and plane strain tests.
This paper proposes slip line fields for bending of unequally notched specimens in plane strain, that have a sharp crack in one side and a sharp V-notch in the other side.
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