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The model is designed to address various research problems, ranging from the specimen origin and type, through cancer development stages to expression patterns.
The MAdL as a marker with sensitivity in between napsin A and SP-A or SP-B will add a decent advantage based on its specificity and persistent expression in terms of grading stages or specimen origin.
Patients records were screened for gender, age, potential mechanism of bacterial access to the bone, time of evolution of COM, bone involved, surgical access to take the bone specimen, origin of specimens cultured, genera and species of organisms identified, and antibiograms (Vitek Systems®, bioMerieux, Hazelwood, MO, USA).
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Detailed information about the specimens' origin is provided in Table 3. Melanoma cell lines (Ma-Mel-46, Ma-Mel-52, Ma-Mel-54a, Ma-Mel-59a) could be established from four of these specimens (Table 4).
Clinical laboratories in Colombia submit isolates with associated information including geographic origin, specimen source, age, sex, and clinical diagnosis of the patient.
So genetic data is not dissociated from the individual, but every sequence can be assigned to its specimen of origin and vice versa.
Finally, DNA barcode libraries are fully available as they are deposited in a major sequence database, and attached to a voucher specimen whose origin and current location are recorded [2], [3].
Our laboratory previously established a procedure [ 29] to derive EC of high purity from human specimens, their origin assessed by immunohistochemical staining for the three endothelial markers CD31/PECAM-1, VE-cadherin and vWF.
This sample volume surge was expected to be specimens of human origin (clinical specimens); the need for large-scale environmental sampling and testing had not been anticipated.
In addition, we also found one specimen where the origin seemed to be at the same place as the LCL.
Table 1 [see Additional file 1] lists specimen information, geographic origin of the specimens, names of collectors, and GenBank accession numbers.
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