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In addition, whether the notification was the result of MRSA isolates found in a clinical specimen or from a screening specimen was recorded.
Total RNA from microscopically confirmed homogeneous breast cancer tissue was isolated from approximately 500 mg of frozen specimen or from one flask of the HepG2 cell line, kindly provided by Dr N Harada [ 21], as a positive control and to generate standard curves.
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In patients with high suspicion for Legionnaires' disease despite negative urinary antigen, additional microbiological tests (culture results from respiratory specimen or PCR from respiratory specimen) were performed based on the discretion of the treating physician.
Data may be obtained from discrete specimens or from continuous measurements.
Total RNA (Table 1) was isolated from original specimens or from suckling mice brain after early passage using Tri-Reagent (Euromedex).
Most quantitative studies of molt come from the examination of museum specimens or from individuals captured during the molt and scored for feather replacement.
All sera were confirmed DENV-2 positive by virus isolation or RT-PCR either from these specimens or from paired acute-phase sera (data not presented and collected in either Puerto Rico or Taiwan).
Total cell lysates were obtained from homogenised tumour specimens or from cells.
Infections were defined as >105 colony-forming units (cfu) grown from either PC specimens or from peripheral blood cultures.
Total RNA was isolated from biopsy and surgical specimens, or from cultured cells, using Trizol reagent (Invitrogen, San Diego, CA, USA).
TBT-PAR was an effective additive in improving PCR amplification of DNA from plant specimens or from old and potentially degraded museum specimens.
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